Dim Source As String = "
Строка для транслитерации"
Source = Source.Replace("а", "a")
Source = Source.Replace("б", "b")
Source = Source.Replace("в", "v")
Source = Source.Replace("г", "g")
Source = Source.Replace("д", "d")
Source = Source.Replace("е", "e")
Source = Source.Replace("ё", "yo")
Source = Source.Replace("ж", "zh")
Source = Source.Replace("з", "z")
Source = Source.Replace("и", "i")
Source = Source.Replace("к", "k")
Source = Source.Replace("л", "l")
Source = Source.Replace("м", "m")
Source = Source.Replace("н", "n")
Source = Source.Replace("о", "o")
Source = Source.Replace("п", "p")
Source = Source.Replace("р", "r")
Source = Source.Replace("с", "s")
Source = Source.Replace("т", "t")
Source = Source.Replace("у", "u")
Source = Source.Replace("ф", "f")
Source = Source.Replace("х", "kh")
Source = Source.Replace("ц", "ts")
Source = Source.Replace("ч", "ch")
Source = Source.Replace("ш", "sh")
Source = Source.Replace("щ", "shch")
Source = Source.Replace("ы", "y")
Source = Source.Replace("э", "a")
Source = Source.Replace("ю", "yu")
Source = Source.Replace("я", "ya")
Source = Source.Replace("ь", "")
Source = Source.Replace("ъ", "")
Source = Source.Replace("А", "A")
Source = Source.Replace("Б", "B")
Source = Source.Replace("В", "V")
Source = Source.Replace("Г", "G")
Source = Source.Replace("Д", "D")
Source = Source.Replace("Е", "E")
Source = Source.Replace("Ё", "Yo")
Source = Source.Replace("Ж", "Zh")
Source = Source.Replace("З", "Z")
Source = Source.Replace("И", "I")
Source = Source.Replace("К", "K")
Source = Source.Replace("Л", "L")
Source = Source.Replace("М", "M")
Source = Source.Replace("Н", "N")
Source = Source.Replace("О", "O")
Source = Source.Replace("П", "P")
Source = Source.Replace("Р", "R")
Source = Source.Replace("С", "S")
Source = Source.Replace("Т", "T")
Source = Source.Replace("У", "U")
Source = Source.Replace("Ф", "F")
Source = Source.Replace("Х", "Kh")
Source = Source.Replace("Ц", "Ts")
Source = Source.Replace("Ч", "Ch")
Source = Source.Replace("Ш", "Sh")
Source = Source.Replace("Щ", "Shch")
Source = Source.Replace("Ы", "Y")
Source = Source.Replace("Э", "A")
Source = Source.Replace("Ю", "Yu")
Source = Source.Replace("Я", "Ya")
Source = Source.Replace("Ь", "")
Source = Source.Replace("Ъ", "")
Label1.Text = Source
вот как-то так...